Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells

Meera Surendran-Nair; Anup Kollanoor-Johny; Sangeetha Ananda-Baskaran; Carol Norris; Ji Young Lee; Kumar Venkitanarayanan

doi:10.2217/fmb.16.16

Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells

Meera Surendran-Nair, Anup Kollanoor-Johny, Sangeetha Ananda-Baskaran, Carol Norris, Ji Young Lee, Kumar Venkitanarayanan

Animal Science

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9 Scopus citations

Abstract

Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.

Original language	English (US)
Pages (from-to)	745-756
Number of pages	12
Journal	Future Microbiology
Volume	11
Issue number	6
DOIs	https://doi.org/10.2217/fmb.16.16
State	Published - Jun 2016

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© 2016 Future Medicine Ltd.

Keywords

E. coli O157:H7
Gb3 receptor
selenium
verotoxin

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title = "Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells",

abstract = "Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.",

keywords = "E. coli O157:H7, Gb3 receptor, selenium, verotoxin",

author = "Meera Surendran-Nair and Anup Kollanoor-Johny and Sangeetha Ananda-Baskaran and Carol Norris and Lee, {Ji Young} and Kumar Venkitanarayanan",

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year = "2016",

month = jun,

doi = "10.2217/fmb.16.16",

language = "English (US)",

volume = "11",

pages = "745--756",

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AU - Surendran-Nair, Meera

AU - Kollanoor-Johny, Anup

AU - Ananda-Baskaran, Sangeetha

AU - Norris, Carol

AU - Lee, Ji Young

AU - Venkitanarayanan, Kumar

PY - 2016/6

Y1 - 2016/6

N2 - Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.

AB - Aim: This study investigated the efficacy of selenium (Se) in reducing Escherichia coli O157:H7 verotoxin production and toxin gene expression. Additionally, the effect of Se on globotriaosylceramide (Gb3) receptor in human lymphoma cells was determined. Materials & methods: The effect of Se on verotoxin synthesis was determined by standard ELISA, whereas its effect on Gb3 receptor was determined by flow cytometry and real-time quantitative PCR. Results & conclusions: Se reduced extracellular and intracellular verotoxin concentration by 40-60% and 80-90%, respectively (p < 0.05), and downregulated verotoxin genes (p < 0.05). Se reduced Gb3 receptor synthesis in lymphoma cells, and real-time quantitative PCR data revealed a significant downregulation of LacCer synthase gene (GalT2) involved in Gb3 synthesis. Further studies are warranted to validate these results in an appropriate animal model.

KW - E. coli O157:H7

KW - Gb3 receptor

KW - selenium

KW - verotoxin

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Selenium reduces enterohemorrhagic Escherichia coli O157:H7 verotoxin production and globotriaosylceramide receptor expression on host cells

Abstract

Bibliographical note

Keywords

UN SDGs

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