Self-assembly of antibodies by chemical induction

Qing Li; David Hapka; Hua Chen; Daniel A. Vallera; Carston R. Wagner

doi:10.1002/anie.200803507

Self-assembly of antibodies by chemical induction

Qing Li, David Hapka, Hua Chen, Daniel A. Vallera, Carston R. Wagner

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

(Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).

Original language	English (US)
Pages (from-to)	10179-10182
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	47
Issue number	52
DOIs	https://doi.org/10.1002/anie.200803507
State	Published - Dec 15 2008

Keywords

Antibiotics
Antibodies
Diabodies
Macrocyclization
Self-assembly

Access

10.1002/anie.200803507

OpenUrl availability

Full text

Cite this

@article{31e55c7a891948b082119a2adba81dfe,

title = "Self-assembly of antibodies by chemical induction",

abstract = "(Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).",

keywords = "Antibiotics, Antibodies, Diabodies, Macrocyclization, Self-assembly",

author = "Qing Li and David Hapka and Hua Chen and Vallera, {Daniel A.} and Wagner, {Carston R.}",

year = "2008",

month = dec,

day = "15",

doi = "10.1002/anie.200803507",

language = "English (US)",

volume = "47",

pages = "10179--10182",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "52",

}

TY - JOUR

T1 - Self-assembly of antibodies by chemical induction

AU - Li, Qing

AU - Hapka, David

AU - Chen, Hua

AU - Vallera, Daniel A.

AU - Wagner, Carston R.

PY - 2008/12/15

Y1 - 2008/12/15

N2 - (Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).

AB - (Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).

KW - Antibiotics

KW - Antibodies

KW - Diabodies

KW - Macrocyclization

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=57749097107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57749097107&partnerID=8YFLogxK

U2 - 10.1002/anie.200803507

DO - 10.1002/anie.200803507

M3 - Article

C2 - 19025747

AN - SCOPUS:57749097107

SN - 1433-7851

VL - 47

SP - 10179

EP - 10182

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 52

ER -

Self-assembly of antibodies by chemical induction

Abstract

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this