Self-assembly of antibodies by chemical induction

Qing Li, David Hapka, Hua Chen, Daniel A. Vallera, Carston R. Wagner

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

(Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).

Original languageEnglish (US)
Pages (from-to)10179-10182
Number of pages4
JournalAngewandte Chemie - International Edition
Volume47
Issue number52
DOIs
StatePublished - Dec 15 2008

Keywords

  • Antibiotics
  • Antibodies
  • Diabodies
  • Macrocyclization
  • Self-assembly

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