Separation characteristics of alkylated guanines in high-performance liquid chromatography

Weiling Xue, Robert M Carlson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O6-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.

Original languageEnglish (US)
Pages (from-to)81-90
Number of pages10
JournalJournal of Chromatography A
Volume447
Issue numberC
DOIs
StatePublished - 1988

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