Separation characteristics of alkylated guanines in high-performance liquid chromatography

Weiling Xue; Robert M Carlson

doi:10.1016/0021-9673(88)90008-8

Separation characteristics of alkylated guanines in high-performance liquid chromatography

Weiling Xue, Robert M Carlson

Chemistry & Biochemistry

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4 Scopus citations

Abstract

The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O⁶-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.

Original language	English (US)
Pages (from-to)	81-90
Number of pages	10
Journal	Journal of Chromatography A
Volume	447
Issue number	C
DOIs	https://doi.org/10.1016/0021-9673(88)90008-8
State	Published - 1988

Bibliographical note

Funding Information:
We thank Mr. R. Liukkonen for helpful discussions and for technical assistance. This work was supported in part by the United States Environmental Protection Agency (CR 813144-02 and CR 813943).

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title = "Separation characteristics of alkylated guanines in high-performance liquid chromatography",

abstract = "The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O6-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.",

author = "Weiling Xue and Carlson, {Robert M}",

note = "Funding Information: We thank Mr. R. Liukkonen for helpful discussions and for technical assistance. This work was supported in part by the United States Environmental Protection Agency (CR 813144-02 and CR 813943).",

year = "1988",

doi = "10.1016/0021-9673(88)90008-8",

language = "English (US)",

volume = "447",

pages = "81--90",

journal = "Journal of Chromatography A",

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T1 - Separation characteristics of alkylated guanines in high-performance liquid chromatography

AU - Xue, Weiling

AU - Carlson, Robert M

N1 - Funding Information: We thank Mr. R. Liukkonen for helpful discussions and for technical assistance. This work was supported in part by the United States Environmental Protection Agency (CR 813144-02 and CR 813943).

PY - 1988

Y1 - 1988

N2 - The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O6-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.

AB - The retention behavior of thirteen alkylated guanines on normal-phase silica gel and amino columns and on reversed-phase ODS and phenyl columns was studied. The larger the alkyl substituent at the same position of guanine the weaker was the retention in the normal-phase chromatographic system and the greater the retention during reversed-phase chromatography. O6-Derivatives possess the lowest polarity in each set of isomers. An amino column was found to be of highest efficiency in terms of separation of the set of ethylguanine isomers and of benzylguanines studied. A phenyl column provided the best resolution of methylated guanines.

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - C

ER -

Separation characteristics of alkylated guanines in high-performance liquid chromatography

Abstract

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