Abstract
Clonazepam, its nitro-reduced metabolites, and the 3-hydroxyl-ated derivatives, were separated by reverse-phase hplc using a four solvent optimization procudure. The separation was used to quantitate the metabolism of3H-clonazepam in rat liver microsomes under aerobic or anaerobic conditions by collection of HPLC eluents and liquid scintillation counting. The predominant metabolite in aerobic incubations was 3-hydroxyclonazepam. The primary anaerobic metabolite was 7-aminoclonazepam. The identity of the microsomal metabolites was confirmed by chemical ionization mass spectrometry. This separation could be applicable for future metabolic studies of clonazepam.
Original language | English (US) |
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Pages (from-to) | 585-598 |
Number of pages | 14 |
Journal | Journal of Liquid Chromatography |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 1983 |
Bibliographical note
Funding Information:We acknowledge support of this work by the NIGMS National Research Service Award GM-07750 to R. P. Remmel. We also wish to thank Larry Oliver for his able technical assistance.