Separation of clonazepam and five metabolites by reverse phase HPLC and quantitation from rat liver microsomal incubations

Rory P. Remmel, Gary W. Elmer

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Clonazepam, its nitro-reduced metabolites, and the 3-hydroxyl-ated derivatives, were separated by reverse-phase hplc using a four solvent optimization procudure. The separation was used to quantitate the metabolism of3H-clonazepam in rat liver microsomes under aerobic or anaerobic conditions by collection of HPLC eluents and liquid scintillation counting. The predominant metabolite in aerobic incubations was 3-hydroxyclonazepam. The primary anaerobic metabolite was 7-aminoclonazepam. The identity of the microsomal metabolites was confirmed by chemical ionization mass spectrometry. This separation could be applicable for future metabolic studies of clonazepam.

Original languageEnglish (US)
Pages (from-to)585-598
Number of pages14
JournalJournal of Liquid Chromatography
Volume6
Issue number3
DOIs
StatePublished - Mar 1 1983

Bibliographical note

Funding Information:
We acknowledge support of this work by the NIGMS National Research Service Award GM-07750 to R. P. Remmel. We also wish to thank Larry Oliver for his able technical assistance.

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