Abstract
Doxorubicinol (DOXol) is a human metabolite of the chemotherapy agent doxorubicin (DOX), and is associated with dose-dependent cardiotoxicity and decreased drug efficacy. Due to the structural similarities and equal molecular charges of DOXol and DOX, their electrophoretic separation is commonly ineffective. A method for separating and detecting DOX and DOXol, as well as two DOXol enantiomers, was established using cyclodextrin-modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Differential DOXol production was detected in a DOX-sensitive and resistant pair of cell lines, with a 0.08±0.01 fmol limit of detection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3263-3270 |
| Number of pages | 8 |
| Journal | ELECTROPHORESIS |
| Volume | 27 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 2006 |
Keywords
- Cyclodextrin
- Doxorubicin
- Doxorubicinol
- Laser-induced fluorescence
- MEKC