Sequence analysis of a mosquito ribosomal protein rpL8 gene and its upstream regulatory region

Que Lan, Ann M Fallon

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The gene encoding Aedes albopictus ribosomal protein L8 was isolated using a cDNA probe. Based on the deduced amino acid sequence, rpL8 has a mass of 28,605 Da, a pl of 11.97, and contains 9.6% Arg and 11.9% Lys. The rpL8 gene spans 1229 nucleotides, and contains three exons measuring 73, 150, and 648 nucleotides. The first intron is 293 nucleotides long and interrupts an 85‐nucleotide untranslated leader sequence. The AUG codon is located 12 nucleotides downstream of the 5′‐end of the second exon. Separating the second and third exons is a 65‐nucleotide intron. The major transcription initiation site, identified by primer extension and polymerase stop reactions, mapped 378 nucleotides upstream from the AUG start codon; minor initiation sites were also detected. The DNA sequence upstream of the rpL8 gene was T‐rich, but conventional TATA and CAAT boxes were absent. This is the first molecular analysis of a mosquito ribosomal protein gene.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalInsect molecular biology
Volume1
Issue number2
DOIs
StatePublished - Nov 1992

Keywords

  • Aedes albopictus
  • gene transfer
  • mosquito
  • reproduction
  • ribosomal protein gene.

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