Sequential G- to R-banding for high resolution chromosome analysis

Betsy Hirsch, Rosalie Mack, Diane Arthur

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

Original languageEnglish (US)
Pages (from-to)37-39
Number of pages3
JournalHuman Genetics
Volume76
Issue number1
DOIs
StatePublished - May 1 1987

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