Sexual recombination in Aspergillus tubingensis

Rodrigo A. Olarte; Bruce W. Horn; Rakhi Singh; Ignazio Carbone

doi:10.3852/14-233

Sexual recombination in Aspergillus tubingensis

Rodrigo A. Olarte, Bruce W. Horn, Rakhi Singh, Ignazio Carbone

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.

Original language	English (US)
Pages (from-to)	307-312
Number of pages	6
Journal	Mycologia
Volume	107
Issue number	2
DOIs	https://doi.org/10.3852/14-233
State	Published - 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 by The Mycological Society of America.

Keywords

Aspergillus niger
Mating type
Multilocus sequence typing
Sexual reproduction

Access

10.3852/14-233

OpenUrl availability

Full text

Cite this

@article{6945ee9a35ba457c9cefa89601011346,

title = "Sexual recombination in Aspergillus tubingensis",

abstract = "Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.",

keywords = "Aspergillus niger, Mating type, Multilocus sequence typing, Sexual reproduction",

author = "Olarte, {Rodrigo A.} and Horn, {Bruce W.} and Rakhi Singh and Ignazio Carbone",

note = "Publisher Copyright: {\textcopyright} 2015 by The Mycological Society of America.",

year = "2015",

doi = "10.3852/14-233",

language = "English (US)",

volume = "107",

pages = "307--312",

journal = "Mycologia",

issn = "0027-5514",

publisher = "Allen Press Inc.",

number = "2",

}

TY - JOUR

T1 - Sexual recombination in Aspergillus tubingensis

AU - Olarte, Rodrigo A.

AU - Horn, Bruce W.

AU - Singh, Rakhi

AU - Carbone, Ignazio

PY - 2015

Y1 - 2015

N2 - Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.

AB - Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.

KW - Aspergillus niger

KW - Mating type

KW - Multilocus sequence typing

KW - Sexual reproduction

UR - http://www.scopus.com/inward/record.url?scp=84938931601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938931601&partnerID=8YFLogxK

U2 - 10.3852/14-233

DO - 10.3852/14-233

M3 - Article

C2 - 25572097

AN - SCOPUS:84938931601

SN - 0027-5514

VL - 107

SP - 307

EP - 312

JO - Mycologia

JF - Mycologia

IS - 2

ER -

Sexual recombination in Aspergillus tubingensis

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this