TY - JOUR
T1 - Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei
AU - Leonardo, Michael R.
AU - Moser, Duane P.
AU - Barbieri, Elena
AU - Brantner, Christine A.
AU - MacGregor, Barbara J.
AU - Paster, Bruce J.
AU - Stackebrandt, Erko
AU - Nealson, Kenneth H.
PY - 1999/10
Y1 - 1999/10
N2 - A new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1(T) (T = type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid Loligo pealei. It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2.0-3.0 μm long, 0.4-0.6 μm wide). ANG-SQ1(T) grows optimally over the temperature range of 25-30 °C and a pH range of 6.5-7.5 °C in media containing 0.5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the γ-3 subclass of the Proteobacteria. The closest relative of ANG-SQ1(T) is Shewanella gelidimarina, with a 16S rRNA sequence similarity of 97.0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine-N-oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG- SQ1(T) was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1T is a Shewanella species. Thus it is proposed that this new isolate be assigned to the genus Shewanella and that it should be named Shewanella pealeana sp. nov., in recognition of its association with L, pealei.
AB - A new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1(T) (T = type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid Loligo pealei. It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2.0-3.0 μm long, 0.4-0.6 μm wide). ANG-SQ1(T) grows optimally over the temperature range of 25-30 °C and a pH range of 6.5-7.5 °C in media containing 0.5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the γ-3 subclass of the Proteobacteria. The closest relative of ANG-SQ1(T) is Shewanella gelidimarina, with a 16S rRNA sequence similarity of 97.0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine-N-oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG- SQ1(T) was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1T is a Shewanella species. Thus it is proposed that this new isolate be assigned to the genus Shewanella and that it should be named Shewanella pealeana sp. nov., in recognition of its association with L, pealei.
KW - Choline
KW - Loligo pealei
KW - Shewanella pealeana
KW - Sulfur reducer
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U2 - 10.1099/00207713-49-4-1341
DO - 10.1099/00207713-49-4-1341
M3 - Article
C2 - 10555311
AN - SCOPUS:0032704458
SN - 0020-7713
VL - 49
SP - 1341
EP - 1351
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
IS - 4
ER -