Short term effects of oxidized ascorbic acid on bovine corneal endothelium and human placenta

Richard C. Rose, Jee Lim Choi, Ann M. Bode

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Studies on the toxic effects of dehydro-L-ascorbic acid (DHAA) have been extended to include evaluations over time periods up to 3 hr. and to test for specific effects on a membrane transport protein, a membrane-bound enzyme and a soluble intracellular enzyme. In studies on cultured corneal endothelial cells, DHAA concentrations of 1, 2, and 5 mM over 3 hr. had an inhibitory effect on subsequent uptake of DHAA present at a tracer level. Surviving fragments of human placenta and alkaline phosphatase activity of the placental brush-border membrane were susceptible to the effect of DHAA at a high concentration (10 mM). Because intracellular metabolism of DHAA was not affected, and an increase in membrane permeability was not detected, it is concluded that a specific membrane transport protein might be the site of DHAA-induced damage. These studies support the concept that the oxidized form of ascorbic acid (vitamin C) has potential toxic effects on biological systems and suggests that proteins that mediate transport and metabolism may be sites where DHAA causes damage.

Original languageEnglish (US)
Pages (from-to)1543-1549
Number of pages7
JournalLife Sciences
Volume50
Issue number20
DOIs
StatePublished - 1992

Bibliographical note

Funding Information:
This work was supported in part by BRSG support to AMB and NIH grants EY 07320 and HD 20398 to RCR.

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