Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells

Soo Hwa Jang; Changsun Choi; Seong Geun Hong; Oleg V. Yarishkin; Young Min Bae; Jae Gon Kim; Scott M. O'Grady; Kyong Ah Yoon; Kyung Sun Kang; Pan Dong Ryu; So Yeong Lee

doi:10.1016/j.bbrc.2009.04.108

Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells

Soo Hwa Jang, Changsun Choi, Seong Geun Hong, Oleg V. Yarishkin, Young Min Bae, Jae Gon Kim, Scott M. O'Grady, Kyong Ah Yoon, Kyung Sun Kang, Pan Dong Ryu, So Yeong Lee

Animal Science

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelial cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation.

Original language	English (US)
Pages (from-to)	180-186
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	384
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2009.04.108
State	Published - Jun 26 2009

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund; KRF-2007-531-E00071) to S.Y.L.

Keywords

Breast cancer
Cell growth
Voltage-gated K channels

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Jang, S. H., Choi, C., Hong, S. G., Yarishkin, O. V., Bae, Y. M., Kim, J. G., O'Grady, S. M., Yoon, K. A., Kang, K. S., Ryu, P. D., & Lee, S. Y. (2009). Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells. Biochemical and Biophysical Research Communications, 384(2), 180-186. https://doi.org/10.1016/j.bbrc.2009.04.108

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abstract = "Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelial cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation.",

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AU - Kang, Kyung Sun

AU - Ryu, Pan Dong

AU - Lee, So Yeong

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N2 - Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelial cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation.

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Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells

Abstract

Bibliographical note

Keywords

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