Similarity between human and chicken Escherichia coli isolates in relation to ciprofloxacin resistance status

James R. Johnson, Michael A. Kuskowski, Megan Menard, Abby Gajewski, Mariona Xercavins, Javier Garau

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

Background. The food supply is suspected to be a source of fluoroquinolone-resistant Escherichia coli that cause disease in humans, but supporting molecular data are lacking. Methods. We performed a molecular-epidemiological comparison, in Barcelona, Spain (1996-1998), of 117 contemporaneous, geographically matched E. coli isolates from humans (35 blood isolates and 33 fecal) or chickens (49 fecal) that were either susceptible (n = 57) or resistant (n = 60) to ciprofloxacin and analyzed them by phylogenetic group, virulence genotype, and O antigens using random amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). Results. When analyzed by phylogenetic distribution, virulence profiles, and O antigens, resistant human isolates were distinct from susceptible human isolates but were largely indistinguishable from chicken isolates, whereas resistant and susceptible chicken isolates were similar. Susceptible human isolates contained more virulence-associated genes and more frequently expressed virulence-associated O antigens than did resistant human or any chicken isolates. Certain resistant human isolates closely resembled chicken isolates by RAPD and PFGE analysis. Conclusions. Ciprofloxacin-resistant E. coli may arise de novo in poultry from susceptible progenitors, be transmitted to humans via the food supply, and go on to cause potentially life-threatening infections. If confirmed, these findings would mandate efforts to eliminate this reservoir of drug-resistant pathogens and/or to block their transmission to humans.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalJournal of Infectious Diseases
Volume194
Issue number1
DOIs
StatePublished - Jul 1 2006

Bibliographical note

Funding Information:
Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs; Centers for Disease Control and Prevention (grant R01-CI000204); National Research Initiative Competitive Grants Program/ United States Department of Agriculture (grant 00-35212-9408) (all to J.R.J.).

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