Simultaneous determination of lipophilic aldehydes by high-performance liquid chromatography in vegetable oil

C. M. Seppanen, A. Saari Csallany

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


A very sensitive high-performance liquid chromatography (HPLC) method was developed for the simultaneous separation and measurement of nonpolar and polar lipophilic secondary lipid peroxidation products in vegetable oil. Seventeen nonpolar and 13 polar lipophilic aldehydes and related carbonyl compounds, derived from thermally oxidized soybean oil as 2,4-dinitrophenyl hydrazones, were separated simultaneously by reversed-phase HPLC. Detection limit for the individual compound is 1 ng. Thirteen of the nonpolar carbonyl compounds were identified as: butanal, 2-butanone, pentanal, 2-pentanone, hexenal, hexanal, 2,4-heptadienal, 2-heptenal, octanal, 2-nonenal, 2,4-decadienal, decanal, and undecanal. Three of the polar carbonyl compounds were identified as: 4-hydroxy-2-hexenal, 4-hydroxy-2-octenal, and 4-hydroxy-2-nonenal. The detection of the toxic 4-hydroxy-2-nonenal, a major compound, and 4-hydroxy-2-hexenal, a minor compound, in heated soybean oil is of particular importance because these toxic compounds have been shown to be absorbed from the diet.

Original languageEnglish (US)
Article number422
Pages (from-to)1253-1260
Number of pages8
JournalJAOCS, Journal of the American Oil Chemists' Society
Issue number12
StatePublished - 2001

Bibliographical note

Funding Information:
This research has been supported in whole or in part by the Minnesota Agricultural Experiment Station.

Copyright 2017 Elsevier B.V., All rights reserved.


  • 4-Hydroxyhexenal
  • 4-Hydroxynonenal
  • 4-Hydroxyoctenal
  • HPLC
  • Heated soybean oil
  • Lipid oxidation
  • Lipophilic aldehydes
  • Secondary oxidation products
  • Toxic aldehydes
  • Vegetable oil

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