TY - JOUR
T1 - Single cell gel electrophoresis assay
T2 - A new technique for human biomonitoring studies
AU - Kassie, Fekadu
AU - Parzefall, Wolfram
AU - Knasmüller, Siegfried
N1 - Funding Information:
This work was supported by a European Community grant to S.K.
PY - 2000/6
Y1 - 2000/6
N2 - Human biomonitoring using the single cell gel electrophoresis (SCGE) or comet assay is a novel approach for the assessment of genetic damage in exposed populations. This assay enables the detection of various forms of DNA damage in individual cells with ease and speed and is, therefore, well suited to the analysis of a large group in a population. Here, application of SCGE assay in the identification of dietary protective factors, in clinical studies and in monitoring the risk of DNA damage resulting from occupational, environmental or lifestyle exposures is reviewed. Also, the comparative sensitivity of SCGE assay and conventional cytogenetic tests to detect genetic damage is discussed. Finally, strengths and shortcomings of the SCGE assay are addressed. Copyright (C) 2000 Elsevier Science B.V.
AB - Human biomonitoring using the single cell gel electrophoresis (SCGE) or comet assay is a novel approach for the assessment of genetic damage in exposed populations. This assay enables the detection of various forms of DNA damage in individual cells with ease and speed and is, therefore, well suited to the analysis of a large group in a population. Here, application of SCGE assay in the identification of dietary protective factors, in clinical studies and in monitoring the risk of DNA damage resulting from occupational, environmental or lifestyle exposures is reviewed. Also, the comparative sensitivity of SCGE assay and conventional cytogenetic tests to detect genetic damage is discussed. Finally, strengths and shortcomings of the SCGE assay are addressed. Copyright (C) 2000 Elsevier Science B.V.
KW - Comet assay
KW - Dietary supplementation
KW - Environmetal pollution
KW - Human biomonitoring
KW - Lifestyle
KW - Occupational exposure
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U2 - 10.1016/S1383-5742(00)00041-7
DO - 10.1016/S1383-5742(00)00041-7
M3 - Article
C2 - 10838207
AN - SCOPUS:0034213140
SN - 1383-5742
VL - 463
SP - 13
EP - 31
JO - Mutation Research - Reviews in Mutation Research
JF - Mutation Research - Reviews in Mutation Research
IS - 1
ER -