Single-molecule cut-and-paste surface assembly

S. K. Kufer, E. M. Puchner, H. Gumpp, T. Liedl, H. E. Gaub

Research output: Contribution to journalArticlepeer-review

221 Scopus citations

Abstract

We introduce a method for the bottom-up assembly of biomolecular structures that combines the precision of the atomic force microscope (AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut-and-paste events was characterized by single-molecule force spectroscopy and single-molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency.

Original languageEnglish (US)
Pages (from-to)594-596
Number of pages3
JournalScience
Volume319
Issue number5863
DOIs
StatePublished - Feb 1 2008

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