Single nucleotide polymorphism analysis indicates genetic distinction and reduced diversity of swine-associated methicillin resistant staphylococcus aureus(MRSA) ST5 isolates compared to clinical MRSA ST5 isolates

Samantha J. Hau, Anna Allué-Guardia, Brigida Rusconi, Jisun S. Haan, Peter R. Davies, Timothy S. Frana, Mark Eppinger, Tracy L. Nicholson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Livestock associated methicillin resistant S. Aureus (LA-MRSA) are lineages adapted to livestock species. LA-MRSA can be transmitted to humans and public health concerns exist because livestock may be the largest MRSA reservoir outside of hospital settings. Although the predominant European (ST398) and Asian (ST9) lineages of LA-MRSA are considered livestock adapted, North American swine also harbor ST5, a globally disseminated and highly pathogenic lineage. This study applied whole genome sequencing and single nucleotide polymorphism (SNP) typing to compare the population structure and genetic relatedness between swine associated and human clinical MRSA ST5 isolates. The established high-resolution phylogenomic framework revealed that LA-MRSA and human clinical MRSA ST5 are genetically distinct. LA-MRSA isolates were found to be clonal within farms, while greater genome diversity was observed among sampled clinical MRSA ST5. Analysis of the accessory genome demonstrated that LA-MRSA ST5 isolates and clinical MRSA ST5 isolates harbor different AMR genes and virulence factors, consistent with the SNP analysis. Collectively, our data indicate LA-MRSA and clinical MRSA ST5 isolates are distinct and the swine reservoir is likely of minimal significance as a source of clinical MRSA ST5 infections.

Original languageEnglish (US)
Article number2078
JournalFrontiers in Microbiology
Volume9
Issue numberSEP
DOIs
StatePublished - Sep 11 2018

Bibliographical note

Funding Information:
This research was conducted in part with funding from the National Pork Board and the United States Department of Agriculture, Agriculture Research Service.

Publisher Copyright:
© 2007-2018 Frontiers Media S.A. All Rights Reserved.

Keywords

  • Agriculture
  • LA-MRSA
  • Mobile genetic elements
  • Phylogenetic analysis
  • Single nucleotide polymorphism (SNP) typing
  • Staphylococcus aureus
  • Swine
  • Whole genome sequence (WGS)

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