Single nucleotide polymorphisms for integrative mapping in the Turkey (Meleagris gallopavo)

K. M. Reed, M. K. Hall, L. D. Chaves, T. P. Knutson

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10 Scopus citations


When multiple genetic maps exist for a species, integration of these maps requires a set of common markers be genotyped across the individual mapping populations. In the turkey, three genetic maps based on separate mapping populations are available. In this study, SNP-based markers were developed for integrating the cDNAIRFLP-based map (1) with microsatellite markers of the second-generation turkey genome map (2). Forty-eight primer sets were designed and tested and 33 (69%) correctly amplified turkey genomic DNA by PCR. Putative SNPs were detected in 20 (61%) of the amplified gene fragments, and 10 SNP markers were subsequently genotyped by PCRIRFLP for segregation analysis. Eight SNP markers were incorporated into the turkey genetic map.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalAnimal Biotechnology
Issue number1
StatePublished - May 2006

Bibliographical note

Funding Information:
This research was supported by grants from the Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture (2001-35205-9494 and 2004-35205-14217).


  • Genetic linkage map
  • Single nucleotide polymorphism
  • Turkey

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