Abstract
When multiple genetic maps exist for a species, integration of these maps requires a set of common markers be genotyped across the individual mapping populations. In the turkey, three genetic maps based on separate mapping populations are available. In this study, SNP-based markers were developed for integrating the cDNAIRFLP-based map (1) with microsatellite markers of the second-generation turkey genome map (2). Forty-eight primer sets were designed and tested and 33 (69%) correctly amplified turkey genomic DNA by PCR. Putative SNPs were detected in 20 (61%) of the amplified gene fragments, and 10 SNP markers were subsequently genotyped by PCRIRFLP for segregation analysis. Eight SNP markers were incorporated into the turkey genetic map.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 73-80 |
| Number of pages | 8 |
| Journal | Animal Biotechnology |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2006 |
Bibliographical note
Funding Information:This research was supported by grants from the Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture (2001-35205-9494 and 2004-35205-14217).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
Keywords
- Genetic linkage map
- Single nucleotide polymorphism
- Turkey