The application of single-quantum-dot-based DNA nanosensor for rapid and highly sensitive detection of DNA for diagnosing genetic diseases was described. The system uses quantum dots linked to DNA probes to capture DNA targets. The target strand binds to a dye-labeled reporter strand thus forming a fluorescence resonance energy transfer (FRET) donor-acceptor ensemble. A nanosensor-based oligonucleotide ligation assay was found to detect a point mutation typical of some ovarian tumors in clinical samples.
Bibliographical noteFunding Information:
The authors thank Y. Peng, S. Yang, S. Lin and Y. P. Ho for valuable discussions, I. M. Shih for providing us with PCR products from clinical samples for Kras point mutation detections, and L. Brand and D. Toptygin for providing support with the TCSPC fluorescence lifetime measurements. This work was supported primarily by NSF under award no. DBI-0352407 and also by the Whitaker Foundation. Correspondence and requests for materials should be addressed to T.-H.W. Supplementary Information accompanies this paper on www.nature.com/naturematerials.