A simple and effective approach is presented for patterning single-walled carbon nanotube (SWNT) microstructures by combining electrostatic layer-by-layer (LbL) nano self-assembly, lithography, and lift-off. This technique provides a simple, low-cost, and low-temperature fabrication method with a short processing time. SWNT micropatterns with feature sizes as small as 5 µm were fabricated and characterized. The thickness of a (PDDA/SWNT) bi-layer was measured approximately as 76 Å. To investigate the potential applications of SWNTs, magnetic cantilever arrays consist of SWNTs, iron oxide (Fe2O3) nanoparticles, and polyelectrolytes were developed. Due to the outstanding mechanical properties of the SWNTs, the fabricated cantilever arrays are strong and flexible. The cantilever arrays can be used in applications such as biosensors and microvalves.