TY - JOUR
T1 - Small heat shock proteins distinguish between two mosquito species and confirm identity of their cell lines
AU - Lan, Q.
AU - Fallon, A. M.
PY - 1990
Y1 - 1990
N2 - We have adapted Aedes aegypti cells to Eagle's minimal medium containing nonessential amino acids, glutamine, and 5% fetal bovine serum. In this medium, cells maintained at 28°C under a 5% CO2 atmosphere had a doubling time of 42 hr. Ninety-three percent of the cells in a typical population contained 6 full-size chromosomes and an apparent chromosome fragment. Ae. aegypti cells could be distinguished from Ae. albopictus cells by the sizes of the small heat shock proteins, whose synthesis was induced by a brief treatment at 41°C. Analysis of the small heat shock proteins synthesized by dissected ovaries from Ae. aegypti and Ae. albopictus confirmed the species of origin of the 2 cell lines.
AB - We have adapted Aedes aegypti cells to Eagle's minimal medium containing nonessential amino acids, glutamine, and 5% fetal bovine serum. In this medium, cells maintained at 28°C under a 5% CO2 atmosphere had a doubling time of 42 hr. Ninety-three percent of the cells in a typical population contained 6 full-size chromosomes and an apparent chromosome fragment. Ae. aegypti cells could be distinguished from Ae. albopictus cells by the sizes of the small heat shock proteins, whose synthesis was induced by a brief treatment at 41°C. Analysis of the small heat shock proteins synthesized by dissected ovaries from Ae. aegypti and Ae. albopictus confirmed the species of origin of the 2 cell lines.
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U2 - 10.4269/ajtmh.1990.43.669
DO - 10.4269/ajtmh.1990.43.669
M3 - Article
C2 - 2267971
AN - SCOPUS:0025602538
SN - 0002-9637
VL - 43
SP - 669
EP - 676
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 6
ER -