Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

Douglas B. Craig; Robert M. Polakowski; Edgar Arriaga; Jerome C Y Wong; Hossein Ahmadzadeh; Costas Stathakis; Norman J. Dovichi

doi:10.1002/elps.1150191222

Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

Douglas B. Craig, Robert M. Polakowski, Edgar Arriaga, Jerome C Y Wong, Hossein Ahmadzadeh, Costas Stathakis, Norman J. Dovichi

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

Original language	English (US)
Pages (from-to)	2175-2178
Number of pages	4
Journal	ELECTROPHORESIS
Volume	19
Issue number	12
DOIs	https://doi.org/10.1002/elps.1150191222
State	Published - Sep 1998

Keywords

Capillary electrophoresis
Protein separation
Sieving matrix
Sodium dodecyl sulfate

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title = "Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection",

abstract = "We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.",

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author = "Craig, {Douglas B.} and Polakowski, {Robert M.} and Edgar Arriaga and Wong, {Jerome C Y} and Hossein Ahmadzadeh and Costas Stathakis and Dovichi, {Norman J.}",

year = "1998",

month = sep,

doi = "10.1002/elps.1150191222",

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volume = "19",

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T1 - Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

AU - Craig, Douglas B.

AU - Polakowski, Robert M.

AU - Arriaga, Edgar

AU - Wong, Jerome C Y

AU - Ahmadzadeh, Hossein

AU - Stathakis, Costas

AU - Dovichi, Norman J.

PY - 1998/9

Y1 - 1998/9

N2 - We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

AB - We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

KW - Capillary electrophoresis

KW - Protein separation

KW - Sieving matrix

KW - Sodium dodecyl sulfate

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VL - 19

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JO - ELECTROPHORESIS

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IS - 12

ER -

Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

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Keywords

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