Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection

Douglas B. Craig, Robert M. Polakowski, Edgar Arriaga, Jerome C Y Wong, Hossein Ahmadzadeh, Costas Stathakis, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.

Original languageEnglish (US)
Pages (from-to)2175-2178
Number of pages4
JournalELECTROPHORESIS
Volume19
Issue number12
DOIs
StatePublished - Sep 1 1998

Keywords

  • Capillary electrophoresis
  • Protein separation
  • Sieving matrix
  • Sodium dodecyl sulfate

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