Solution Conformation of a Cyclophilin-Bound Proline Isomerase Substrate

Lazaros T. Kakalis; Ian M. Armitage

doi:10.1021/bi00172a028

Solution Conformation of a Cyclophilin-Bound Proline Isomerase Substrate

Lazaros T. Kakalis, Ian M. Armitage

Biochemistry, Molecular Biology, and Biophysics (TMED)

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15 Scopus citations

Abstract

Cyclophilin (CyP) is the 17.8-kDa cytosolic receptor of the immunosuppressant cyclosporin A (CsA) and also a peptidyl prolyl cis‒trans isomerase (PPIase). In order to gain insights into the PPIase mechanism, transferred nuclear Overhauser effect (TRNOE) measurements by two-dimensional ¹H NMR were used to determine the conformation of the isomerase-bound standard model substrate suc-AAPF-pNA. Results indicate a cis-like conformation for the CyP-bound substrate with the A‒P peptide bond being no more than 40° out of planarity.

Original language	English (US)
Pages (from-to)	1495-1501
Number of pages	7
Journal	Biochemistry
Volume	33
Issue number	6
DOIs	https://doi.org/10.1021/bi00172a028
State	Published - Feb 1 1994

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abstract = "Cyclophilin (CyP) is the 17.8-kDa cytosolic receptor of the immunosuppressant cyclosporin A (CsA) and also a peptidyl prolyl cis‒trans isomerase (PPIase). In order to gain insights into the PPIase mechanism, transferred nuclear Overhauser effect (TRNOE) measurements by two-dimensional 1H NMR were used to determine the conformation of the isomerase-bound standard model substrate suc-AAPF-pNA. Results indicate a cis-like conformation for the CyP-bound substrate with the A‒P peptide bond being no more than 40° out of planarity.",

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N2 - Cyclophilin (CyP) is the 17.8-kDa cytosolic receptor of the immunosuppressant cyclosporin A (CsA) and also a peptidyl prolyl cis‒trans isomerase (PPIase). In order to gain insights into the PPIase mechanism, transferred nuclear Overhauser effect (TRNOE) measurements by two-dimensional 1H NMR were used to determine the conformation of the isomerase-bound standard model substrate suc-AAPF-pNA. Results indicate a cis-like conformation for the CyP-bound substrate with the A‒P peptide bond being no more than 40° out of planarity.

AB - Cyclophilin (CyP) is the 17.8-kDa cytosolic receptor of the immunosuppressant cyclosporin A (CsA) and also a peptidyl prolyl cis‒trans isomerase (PPIase). In order to gain insights into the PPIase mechanism, transferred nuclear Overhauser effect (TRNOE) measurements by two-dimensional 1H NMR were used to determine the conformation of the isomerase-bound standard model substrate suc-AAPF-pNA. Results indicate a cis-like conformation for the CyP-bound substrate with the A‒P peptide bond being no more than 40° out of planarity.

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Solution Conformation of a Cyclophilin-Bound Proline Isomerase Substrate

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