To investigate the gypsy functional activity, the possibility of conservation of its genetic information in VLP in the extracellular form has been examined. Preparations containing extracellular gypsy VLP from D. melanogaster and D. virilis were obtained. Non degradative full-sized polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were revealed in the both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in VLP preparations. Some morphological and physical characteristics of the VLP preparations were obtained. The data obtained suggest the presence of non-degradative gypsy VLP in cultured media of D. melanogaster and D. virilis cells.
|Translated title of the contribution||Some biochemical characteristics of substances from extracellular virus-like particles of the Gypsy retrotransposon (MDG4)|
|Number of pages||10|
|State||Published - Apr 1 1994|