Spontaneous decay of oxidized ascorbic acid (dehydro-L-ascorbic acid) evaluated by high-pressure liquid chromatography

A. M. Bode, L. Cunningham, R. C. Rose

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165 Scopus citations

Abstract

We applied high-pressure liquid chromatography to assess the decomposition of the oxidized form of vitamin C, dehydro-L-ascorbic acid. We selected experimental conditions that might represent a wide variety of clinical and research procedures. Decay of dehydro-L-ascorbic acid proceeded much more rapidly at high pH (7-8) than at low pH (3-5) and was more rapid at 37 or 45°C than at 0 or 23°C. When evaluated at pH 6.6, the percent decay was somewhat more rapid from an initial concentration of 1000 μmol/L than at 5-10 μmol/L. The analytical procedure (HPLC) provided useful information about the rate of decay under various conditions. This may facilitate future biological and clinical studies that require a distinction between the oxidized and reduced forms of vitamin C.

Original languageEnglish (US)
Pages (from-to)1807-1809
Number of pages3
JournalClinical chemistry
Volume36
Issue number10
DOIs
StatePublished - 1990

Keywords

  • Variation, source of
  • radioassay

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