Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia

Courtney E. Johnson; Davis M. Seelig; Frances M. Moore; Tammy J. Ruska; Daniel A. Heinrich

doi:10.1111/vcp.12860

Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia

Courtney E. Johnson, Davis M. Seelig, Frances M. Moore, Tammy J. Ruska, Daniel A. Heinrich

Veterinary Clinical Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Two domestic shorthair cats were presented with anorexia and dehydration following ingestion of caramelized onions. Shared key findings from a CBC (ADVIA 2120), serum biochemistry, and urinalysis included a spurious, marked leukocytosis with discordant basophil (BASO) channel and peroxidase channel WBC counts, normal manual leukocyte counts, mild, non-regenerative anemia with discrepancies between automated and manual reticulocyte counts, an abundance of large Heinz bodies (HBs), and highly irregular scattergrams. Case 1 also demonstrated a markedly elevated mean corpuscular hemoglobin concentration (MCHC) and discrepancies between RBC hemoglobin indices. Spurious leukocyte results were confirmed through re-analysis of samples (including the acquisition of a new sample, use of an alternate analyzer (Sysmex XT-2000iV; Case 1 only), and evaluation of scattergrams and blood films (Cases 1 and 2). Repeatedly discrepant reticulocyte counts were also identified. In both cases, the erroneous BASO WBC counts, discrepancies in reticulocyte counts and RBC indices, and atypical scattergrams were interpreted to result from various effects of the HBs. These cases emphasize the importance of reviewing blood films, interpreting scattergrams, and the usefulness of duplicate methods for determining various measurands on hematology analyzers.

Original language	English (US)
Pages (from-to)	232-239
Number of pages	8
Journal	Veterinary Clinical Pathology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1111/vcp.12860
State	Published - Jun 1 2020

Bibliographical note

Funding Information:
The authors thank Dr Harold Tvedten (Swedish University of Agricultural Sciences), Dr Julia Ryseff (IDEXX Reference Laboratories), Dr Mary Christopher (University of California-Davis School of Veterinary Medicine), and Dr Tracy Stokol (Cornell University College of Veterinary Medicine) for their input, and to Dr Harold Tvedten for reviewing the manuscript. The authors also thank Dr Erin Burton (University of Minnesota College of Veterinary Medicine) and Dr Angela Royal (University of Missouri College of Veterinary Medicine) for their photo contributions and support.

Publisher Copyright:
© 2020 American Society for Veterinary Clinical Pathology

Keywords

ADVIA
Sysmex
feline
hemolysis
oxidative damage

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title = "Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia",

abstract = "Two domestic shorthair cats were presented with anorexia and dehydration following ingestion of caramelized onions. Shared key findings from a CBC (ADVIA 2120), serum biochemistry, and urinalysis included a spurious, marked leukocytosis with discordant basophil (BASO) channel and peroxidase channel WBC counts, normal manual leukocyte counts, mild, non-regenerative anemia with discrepancies between automated and manual reticulocyte counts, an abundance of large Heinz bodies (HBs), and highly irregular scattergrams. Case 1 also demonstrated a markedly elevated mean corpuscular hemoglobin concentration (MCHC) and discrepancies between RBC hemoglobin indices. Spurious leukocyte results were confirmed through re-analysis of samples (including the acquisition of a new sample, use of an alternate analyzer (Sysmex XT-2000iV; Case 1 only), and evaluation of scattergrams and blood films (Cases 1 and 2). Repeatedly discrepant reticulocyte counts were also identified. In both cases, the erroneous BASO WBC counts, discrepancies in reticulocyte counts and RBC indices, and atypical scattergrams were interpreted to result from various effects of the HBs. These cases emphasize the importance of reviewing blood films, interpreting scattergrams, and the usefulness of duplicate methods for determining various measurands on hematology analyzers.",

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note = "Funding Information: The authors thank Dr Harold Tvedten (Swedish University of Agricultural Sciences), Dr Julia Ryseff (IDEXX Reference Laboratories), Dr Mary Christopher (University of California-Davis School of Veterinary Medicine), and Dr Tracy Stokol (Cornell University College of Veterinary Medicine) for their input, and to Dr Harold Tvedten for reviewing the manuscript. The authors also thank Dr Erin Burton (University of Minnesota College of Veterinary Medicine) and Dr Angela Royal (University of Missouri College of Veterinary Medicine) for their photo contributions and support. Publisher Copyright: {\textcopyright} 2020 American Society for Veterinary Clinical Pathology",

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Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia

Abstract

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Keywords

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