Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus

A. D. Krikorian; H. Irizarry; R. Goenaga; Mary E. Scott; B. E.L. Lockhart

doi:10.1016/S0304-4238(99)00006-0

Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus

A. D. Krikorian, H. Irizarry, R. Goenaga, Mary E. Scott, B. E.L. Lockhart

Plant Pathology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Field evaluation of plants produced in vitro initiated from a phenotypically distinct, apparently healthy dwarf 'French-type' plantain clone 'Dwarf Superplatano' (Musa 'AAB') was carried out at three sites in Puerto Rico. The use of the floral axis tip from a 'mother plant' versus vegetative apices from lateral buds of the same plant as a source of the primary explant was compared and contrasted. Material from floral axis tip consistently showed high phenotypic uniformly whereas materials from vegetative apices of 'sword' suckers were less so. 'Virus-like symptoms' that became apparent in much of the material just before flowering (shooting stage) were determined to be due to the badnavirus banana streak virus (BSV), a dsDNA pararetrovirus. The 'good news' is that a primary explant taken from the floral axis tip was quicker in its initial response to yield a multiplication system in vitro, and produced significantly fewer virus-infected plants, ca. 5%. By contrast, primary explants obtained from the vegetative sucker-derived apices were later in their production of initial buds, and produced many more virus-infected plants, an average of 32%. Comparison of vegetative-apex-derived plants and floral axis tip-derived plants disclosed no evidence that apices from vegetative suckers or floral stem tips gave rise to genetic off-types due to mutations were brought about by the in vitro process per se. Phenotypic differences were due to virus infestation. The 'bad news' is that this dwarf plantain clone of considerable interest and potential in Puerto Rico can show very severe BSV symptoms. The occurrence of BSV infection in tissue culture-derived plants may be related to the presence of viral sequences integrated into the host genome, in which case there is no apparent strategy to rid the clone of this 'virus'.

Original language	English (US)
Pages (from-to)	159-177
Number of pages	19
Journal	Scientia Horticulturae
Volume	81
Issue number	2
DOIs	https://doi.org/10.1016/S0304-4238(99)00006-0
State	Published - Jun 1 1999

Bibliographical note

Funding Information:
This paper covers work carried out cooperatively between the Agricultural Research Service-USDA and the Agricultural Experiment Station, University of Puerto Rico (AES-UPR), Rio Piedras, Puerto Rico and Professor A.D. Krikorian. Earlier parts of the tissue culture work were supported by a grant to A.D. Krikorian from the US Agency for International Development for a project entitled “Tissue Culture for Banana and Plantain Improvement”. Research on occurrence and identification of BSV in Musa was supported by a grant to B.E.L Lockhart from the International Network for the Improvement of Banana and Plantain.

Keywords

Banana streak virus (BSV)
Clonal multiplication
Musa
Phenotype
Plantains
Somaclonal variation
Tissue culture

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Krikorian, A. D., Irizarry, H., Goenaga, R., Scott, M. E., & Lockhart, B. E. L. (1999). Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus. Scientia Horticulturae, 81(2), 159-177. https://doi.org/10.1016/S0304-4238(99)00006-0

Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus. / Krikorian, A. D.; Irizarry, H.; Goenaga, R. et al.
In: Scientia Horticulturae, Vol. 81, No. 2, 01.06.1999, p. 159-177.

Research output: Contribution to journal › Article › peer-review

Krikorian, AD, Irizarry, H, Goenaga, R, Scott, ME & Lockhart, BEL 1999, 'Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus', Scientia Horticulturae, vol. 81, no. 2, pp. 159-177. https://doi.org/10.1016/S0304-4238(99)00006-0

Krikorian AD, Irizarry H, Goenaga R, Scott ME, Lockhart BEL. Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus. Scientia Horticulturae. 1999 Jun 1;81(2):159-177. doi: 10.1016/S0304-4238(99)00006-0

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abstract = "Field evaluation of plants produced in vitro initiated from a phenotypically distinct, apparently healthy dwarf 'French-type' plantain clone 'Dwarf Superplatano' (Musa 'AAB') was carried out at three sites in Puerto Rico. The use of the floral axis tip from a 'mother plant' versus vegetative apices from lateral buds of the same plant as a source of the primary explant was compared and contrasted. Material from floral axis tip consistently showed high phenotypic uniformly whereas materials from vegetative apices of 'sword' suckers were less so. 'Virus-like symptoms' that became apparent in much of the material just before flowering (shooting stage) were determined to be due to the badnavirus banana streak virus (BSV), a dsDNA pararetrovirus. The 'good news' is that a primary explant taken from the floral axis tip was quicker in its initial response to yield a multiplication system in vitro, and produced significantly fewer virus-infected plants, ca. 5%. By contrast, primary explants obtained from the vegetative sucker-derived apices were later in their production of initial buds, and produced many more virus-infected plants, an average of 32%. Comparison of vegetative-apex-derived plants and floral axis tip-derived plants disclosed no evidence that apices from vegetative suckers or floral stem tips gave rise to genetic off-types due to mutations were brought about by the in vitro process per se. Phenotypic differences were due to virus infestation. The 'bad news' is that this dwarf plantain clone of considerable interest and potential in Puerto Rico can show very severe BSV symptoms. The occurrence of BSV infection in tissue culture-derived plants may be related to the presence of viral sequences integrated into the host genome, in which case there is no apparent strategy to rid the clone of this 'virus'.",

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note = "Funding Information: This paper covers work carried out cooperatively between the Agricultural Research Service-USDA and the Agricultural Experiment Station, University of Puerto Rico (AES-UPR), Rio Piedras, Puerto Rico and Professor A.D. Krikorian. Earlier parts of the tissue culture work were supported by a grant to A.D. Krikorian from the US Agency for International Development for a project entitled “Tissue Culture for Banana and Plantain Improvement”. Research on occurrence and identification of BSV in Musa was supported by a grant to B.E.L Lockhart from the International Network for the Improvement of Banana and Plantain. ",

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AU - Irizarry, H.

AU - Goenaga, R.

AU - Scott, Mary E.

AU - Lockhart, B. E.L.

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KW - Phenotype

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Stability in plant and bunch traits of a 'French-type' dwarf plantain micropropagated from the floral axis tip and five lateral corm tips of a single mother plant: Good news on the tissue culture and bad news on banana streak virus

Abstract

Bibliographical note

Keywords

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