TY - JOUR
T1 - Stimulating beta cell replication and improving islet graft function by GPR119 agonists
AU - Gao, Jie
AU - Tian, Lei
AU - Weng, Guobin
AU - Bhagroo, Nicholas V.
AU - Sorenson, Robert L.
AU - O'Brien, Timothy D.
AU - Luo, Jian
AU - Guo, Zhiguang
PY - 2011/11
Y1 - 2011/11
N2 - G protein-coupled receptor 119 (GPR119) is predominantly expressed in β cells and intestinal L cells. In this study, we investigated whether oleoylethanolamide (OEA), a GPR119 endogenous ligand, and PSN632408, a GPR119 synthetic agonist, can stimulate β-cell replication in vitro and in vivo and improve islet graft function in diabetic mice. We found that OEA and PSN632408 significantly increased numbers of insulin +/5-bromo- 2′-deoxyuridine (BrdU) +β cells in cultured mouse islets in a dose-dependent manner. All diabetic recipient mice, given marginal syngeneic islet transplants with OEA or PSN632408 or vehicle, achieved normoglycemia at 4 weeks after transplantation. However, normoglycemia was achieved significantly faster in OEA- or PSN632408-treated diabetic mice than in vehicle-treated diabetic mice (P < 0.05). The percentage of insulin +/BrdU +β cells in islet grafts in OEA- and PSN632408-treated mice was significantly higher than in vehicle-treated mice (P < 0.01). Our data demonstrated that OEA and PSN632408 can stimulate β-cell replication in vitro and in vivo and improve islet graft function. Targeting GPR119 is a novel therapeutic approach to increase β-cell mass and to improve islet graft function by stimulating β-cell replication.
AB - G protein-coupled receptor 119 (GPR119) is predominantly expressed in β cells and intestinal L cells. In this study, we investigated whether oleoylethanolamide (OEA), a GPR119 endogenous ligand, and PSN632408, a GPR119 synthetic agonist, can stimulate β-cell replication in vitro and in vivo and improve islet graft function in diabetic mice. We found that OEA and PSN632408 significantly increased numbers of insulin +/5-bromo- 2′-deoxyuridine (BrdU) +β cells in cultured mouse islets in a dose-dependent manner. All diabetic recipient mice, given marginal syngeneic islet transplants with OEA or PSN632408 or vehicle, achieved normoglycemia at 4 weeks after transplantation. However, normoglycemia was achieved significantly faster in OEA- or PSN632408-treated diabetic mice than in vehicle-treated diabetic mice (P < 0.05). The percentage of insulin +/BrdU +β cells in islet grafts in OEA- and PSN632408-treated mice was significantly higher than in vehicle-treated mice (P < 0.01). Our data demonstrated that OEA and PSN632408 can stimulate β-cell replication in vitro and in vivo and improve islet graft function. Targeting GPR119 is a novel therapeutic approach to increase β-cell mass and to improve islet graft function by stimulating β-cell replication.
KW - GLP-1
KW - GPR119
KW - islet graft function
KW - islet transplantation
KW - β-cell regeneration
KW - β-cell replication
UR - http://www.scopus.com/inward/record.url?scp=80053983857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053983857&partnerID=8YFLogxK
U2 - 10.1111/j.1432-2277.2011.01332.x
DO - 10.1111/j.1432-2277.2011.01332.x
M3 - Article
C2 - 21902730
AN - SCOPUS:80053983857
SN - 0934-0874
VL - 24
SP - 1124
EP - 1134
JO - Transplant International
JF - Transplant International
IS - 11
ER -