Stimulation of in vitro muscle cell proliferation by sera from swine injected with porcine growth hormone.

C. E. Kotts, F. Buonomo, M. E. White, C. E. Allen, W. R. Dayton

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The proliferation-promoting activity of sera obtained from pigs before and after porcine growth hormone injections was tested in a muscle cell culture bioassay. For 3 d, purified porcine growth hormone (pGH) was administered by intramuscular injection to crossbred barrows. Two levels of pGH were administered: 18 micrograms pGH X kg-1 body weight X d-1 (low dose) or 143 micrograms pGH X kg-1 body weight X d-1 (high dose). Multiple blood samples were withdrawn from jugular catheters for 3 d prior to the injection, during the injection period and for 6 d after the last injection. Although serum pGH levels in low-dose pigs were raised from two to three times pre-injection levels, there was no significant change in serum proliferation-promoting activity or somatomedin-C (SmC), insulin or cortisol levels during or after administration of pGH. In contrast, the proliferation-promoting activity of sera obtained during and after the high-dose pGH injections was higher (P less than .005) than the pre-injection levels. Serum pGH levels were increased approximately 30-fold by 4 h after each injection, and increases in SmC levels were observed 10 to 16 h after the pGH injection. During the injection period SmC levels increased from 1.7 to 4 times pre-injection levels. Insulin and cortisol levels did not change significantly during the 3-d treatment period. We believe that this muscle cell culture bioassay system will be a useful addition to traditional radioimmunoassays and whole animal studies in elucidating the mode of action of pGH in pituitary-intact swine.

Original languageEnglish (US)
Pages (from-to)623-632
Number of pages10
JournalJournal of animal science
Volume64
Issue number2
DOIs
StatePublished - Feb 1987

Fingerprint Dive into the research topics of 'Stimulation of in vitro muscle cell proliferation by sera from swine injected with porcine growth hormone.'. Together they form a unique fingerprint.

Cite this