Wolbachia pipientis (Rickettsiales; Anaplasmataceae) is an obligate intracellular alpha proteobacterium that occurs in arthropods and filarial worms. Some strains of Wolbachia can be maintained as persistent infections in insect cell lines. C/wStr1 cells from the mosquito Aedes albopictus maintain a robust infection with Wolbachia strain wStr, originally isolated from the planthopper, Laodelphax striatellus. To explore possible functions of penicillin-binding proteins expressed from the wStr genome, C/wStr1 cells were exposed to ampicillin. Absolute levels of Wolbachia increased 3.5-fold in ampicillin-treated cells and fivefold in naive cells newly infected with wStr. Because cell numbers were depressed by ampicillin treatment, Wolbachia yield on a per-cell basis increased by 15-fold. The absence of a similar effect on wAlbB in Aa23 host cells suggests that the Wolbachia strain, the presence/absence of genes encoding penicillin-binding proteins, or the interaction between wAlbB and its host cells may modulate the effects of ampicillin.
|Original language||English (US)|
|Number of pages||9|
|Journal||In Vitro Cellular and Developmental Biology - Animal|
|State||Published - Sep 1 2018|
Bibliographical noteFunding Information:
Funding information This work was supported by NIH grant AI081322 and by the University of Minnesota Agricultural Experiment Station, St. Paul, MN.
© 2018, The Society for In Vitro Biology.
- Alpha proteobacteria
- Flow cytometry
- Intracellular bacteria
- Mosquito cell lines