Strategy for distinguishing a new DQB 1 allele (DQB1(*)0611) from the closely related DQB 1(*)0602 allele via sequence specific PCR or direct DNA sequencing

T. M. Williams, S. Bassinger, C. Moehlenkamp, J. Wu, G. D. Montoya, B. B. Griffith, J. D. McAuley, S. Goldman, D. H. Maurer, G. M. Troup

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15 Scopus citations

Abstract

A novel DQ6 allele (DQB 1(*)0611) was identified via direct DNA sequencing in an African-American donor for bone marrow transplantation. The allele was not suspected on the basis of a sequence specific PCR assay which instead indicated the presence of DQB1(*)0602. DQB1(*)0602 and DQB1(*)0611 differ in exon 2 only at codon 9 resulting in a tyrosine substitution for phenylalanine. A modification of current DQB 1 sequence specific PCR assays was devised which allows distinction between the closely related DQB1(*)0602 and DQB1(*)0611 alleles. Preliminary allele frequency studies suggest that DQB1(*)0611 is rare both in a non-African American sample and in Americans of African descent carrying DR11, DQ6 haplotypes. The selection of various DQB1(*)0611 detection methods is discussed.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalTissue Antigens
Volume48
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Bone marrow transplantation
  • Direct DNA sequencing
  • HLA-DQB1
  • Sequence specific PCR

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