TY - JOUR
T1 - Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans
AU - Mekasha, Sophanit
AU - Forsberg, Zarah
AU - Dalhus, Bjørn
AU - Bacik, John Paul
AU - Choudhary, Swati
AU - Schmidt-Dannert, Claudia
AU - Vaaje-Kolstad, Gustav
AU - Eijsink, Vincent G.H.
N1 - Publisher Copyright:
© 2015 Federation of European Biochemical Societies.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Lytic polysaccharide monooxygenases (LPMOs) boost enzymatic depolymerization of recalcitrant polysaccharides, such as chitin and cellulose. We have studied a chitin-active LPMO domain (JdLPMO10A) that is considerably smaller (15.5 kDa) than all structurally characterized LPMOs so far and that is part of a modular protein containing a GH18 chitinase. The 1.55 Å resolution structure revealed deletions of interacting loops that protrude from the core β-sandwich scaffold in larger LPMO10s. Despite these deletions, the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved. JdLPMO10A may represent a minimal scaffold needed to catalyse the powerful LPMO reaction.
AB - Lytic polysaccharide monooxygenases (LPMOs) boost enzymatic depolymerization of recalcitrant polysaccharides, such as chitin and cellulose. We have studied a chitin-active LPMO domain (JdLPMO10A) that is considerably smaller (15.5 kDa) than all structurally characterized LPMOs so far and that is part of a modular protein containing a GH18 chitinase. The 1.55 Å resolution structure revealed deletions of interacting loops that protrude from the core β-sandwich scaffold in larger LPMO10s. Despite these deletions, the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved. JdLPMO10A may represent a minimal scaffold needed to catalyse the powerful LPMO reaction.
KW - AA10
KW - Jonesia denitrificans
KW - chitin
KW - chitinase
KW - lytic polysaccharide monooxygenase
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U2 - 10.1002/1873-3468.12025
DO - 10.1002/1873-3468.12025
M3 - Article
C2 - 26763108
AN - SCOPUS:84956633389
SN - 0014-5793
VL - 590
SP - 34
EP - 42
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -