Structural features promoting dioxygen production by Dechloromonas aromatica chlorite dismutase

Brandon R. Goblirsch, Bennett R. Streit, Jennifer L. DuBois, Carrie M. Wilmot

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47 Scopus citations

Abstract

Chlorite dismutase (Cld) is a heme enzyme capable of rapidly and selectively decomposing chlorite (ClO 2 -) to Cl - and O 2. The ability of Cld to promote O 2 formation from ClO 2 - is unusual. Heme enzymes generally utilize ClO 2 - as an oxidant for reactions such as oxygen atom transfer to, or halogenation of, a second substrate. The X-ray crystal structure of Dechloromonas aromatica Cld co-crystallized with the substrate analogue nitrite (NO 2 -) was determined to investigate features responsible for this novel reactivity. The enzyme active site contains a single btype heme coordinated by a proximal histidine residue. Structural analysis identified a glutamate residue hydrogenbonded to the heme proximal histidine that may stabilize reactive heme species. A solvent-exposed arginine residue likely gates substrate entry to a tightly confined distal pocket. On the basis of the proposed mechanism of Cld, initial reaction of ClO 2 - within the distal pocket generates hypochlorite (ClO -) and a compound I intermediate. The sterically restrictive distal pocket probably facilitates the rapid rebound of ClO - with compound I forming the Cl - and O 2 products. Common to other heme enzymes, Cld is inactivated after a finite number of turnovers, potentially via the observed formation of an off-pathway tryptophanyl radical species through electron migration to compound I. Three tryptophan residues of Cld have been identified as candidates for this off-pathway radical. Finally, a juxtaposition of hydrophobic residues between the distal pocket and the enzyme surface suggests O 2 may have a preferential direction for exiting the active site.

Original languageEnglish (US)
Pages (from-to)879-888
Number of pages10
JournalJournal of Biological Inorganic Chemistry
Volume15
Issue number6
DOIs
StatePublished - Aug 2010

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the National Institutes of Health (R01 GM-66569 to C.M.W.; R03 ES-14390 and R01 GM-90260 to J.L.D.), and a Minnesota Partnership for Biotechnology and Medical Genomics grant SPAP-05-0013-P-FY06 to C.M.W. B.R.S. was supported by an Environmental Protection Agency STAR fellowship (FP-91690601-0). Computer resources were provided by the Basic Sciences Computing Laboratory of the University of Minnesota Supercomputing Institute, and we thank Can Ergenekan for his support. X-ray data were collected at the Kahlert Structural Biology Laboratory (KSBL) at The University of Minnesota and beamline 19-ID, Structural Biology Consortium– Collaborative Access Team, at the Advanced Photon Source, Argonne National Laboratory (Argonne, IL, USA). Argonne National Laboratory is operated by University of Chicago Argonne LLC for the US Department of Energy, Office of Biological and Environmental Research under contract DE-AC02-06CH11357. We thank Ed Hoe-ffner for KSBL support and Steve Ginell and the staff at Sector 19, Advanced Photon Source, for their support.

Keywords

  • Chlorite dismutase
  • Crystal structure
  • Heme

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