Structural insights into NusG regulating transcription elongation

Bin Liu, Thomas A. Steitz

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Escherichia coli RNA polymerase complexed with NusG. The structure shows that the NusG N-terminal domain (NGN) binds at the central cleft of RNA polymerase surrounded by the β′ clamp helices, the β protrusion, and the β lobe domains to close the promoter DNA binding channel and constrain the β′ clamp domain, but with an orientation that is different from the one observed in the archaeal β′ clamp-Spt4/5 complex. The structure also allows us to construct a reliable model of the complete NusG-associated transcription elongation complex, suggesting that the NGN domain binds at the upstream fork junction of the transcription elongation complex, similar to σ2 in the transcription initiation complex, to stabilize the junction, and therefore enhances transcription processivity.

Original languageEnglish (US)
Pages (from-to)968-974
Number of pages7
JournalNucleic acids research
Issue number2
StatePublished - Jan 1 2017

Bibliographical note

Funding Information:
We thank Y. Wang for constructing E. coli NusG plasmid. We also thank the staff of the Argonne National Laboratory beamline 24-ID-E for help during data collection and the Center for Structural Biology Facility at Yale University for computational support. B.L. designed and performed the experiments; B.L. and T.A.S. wrote the manuscript. National Institutes of Health [GM22778 to T.A.S.]; T.A.S. is an investigator of the Howard Hughes Medical Institute. Funding for open access charge: National Institutes of Health [GM22778].

Publisher Copyright:
© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Copyright 2018 Elsevier B.V., All rights reserved.

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