Abstract
Compartmentalization of metabolic pathways to particular organelles is a hallmark of eukaryotic cells. Knowledge of the development of organelles and attendant pathways under different metabolic states has been advanced by live cell imaging and organelle specific analysis. Nevertheless, relatively few studies have addressed the cellular localization of pathways for synthesis of fungal secondary metabolites, despite their importance as bioactive compounds with significance to medicine and agriculture. When triggered to produce sesquiterpene (trichothecene) mycotoxins, the endoplasmic reticulum (ER) of the phytopathogenic fungus Fusarium graminearum is reorganized both in vitro and in planta. Trichothecene biosynthetic enzymes accumulate in organized smooth ER with pronounced expansion at perinuclear- and peripheral positions. Fluorescence tagged trichothecene biosynthetic proteins co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. We hypothesize that changes to the fungal ER represent a conserved process in specialized eukaryotic cells such as in mammalian hepatocytes and B-cells.
Original language | English (US) |
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Article number | 44296 |
Journal | Scientific reports |
Volume | 7 |
DOIs | |
State | Published - Mar 13 2017 |
Bibliographical note
Funding Information:This work was funded by award 2014-67013-21561 from the Agriculture and Food Research Initiative of the National Institute of Food and Agriculture, United States Department of Agriculture. FACS and proteomics were performed under the Facilities Integrating Collaborations for User Science initiative and used resources at the Environmental Molecular Sciences Laboratory, which is a DOE Office of Science User Facilities sponsored by the Office of Biological and Environmental Research and operated under Contract DE-AC05-76RL01830. M.F. was funded by NSF grant MCB1515998.
Publisher Copyright:
© The Author(s) 2017.