Structure and function of the B and D genes of the Actinobacillus actinomycetemcomitans leukotoxin complex

Edward T. Lally; Ellis E. Golub; Irene R. Kieba; Norton S. Taichman; Sylvia Decker; Peter Berthold; Carolyn W. Gibson; Donald R. Demuth; Joel Rosenbloom

doi:10.1016/0882-4010(91)90004-T

Structure and function of the B and D genes of the Actinobacillus actinomycetemcomitans leukotoxin complex

Edward T. Lally, Ellis E. Golub, Irene R. Kieba, Norton S. Taichman, Sylvia Decker, Peter Berthold, Carolyn W. Gibson, Donald R. Demuth, Joel Rosenbloom

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Abstract

The Actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the AaLtC and AaLtA genes reported. The present paper details the sequences of the AaLtB and AaLtD genes which, like AaLtC and AaLtA, are also homologues of genes found in other cytolytic toxin complexes of several other Gram-negative bacterial pathogens. When tested in a recombinant expression system, the AaLtB and/or AaLtD genes are required for the translocation and insertion of the A. actinomycetemcomitans leukotoxin (AaLtA) into the cell membrane of Escherichia coli.

Original language	English (US)
Pages (from-to)	111-121
Number of pages	11
Journal	Microbial Pathogenesis
Volume	11
Issue number	2
DOIs	https://doi.org/10.1016/0882-4010(91)90004-T
State	Published - Aug 1991
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health grants DE07118, DE08239 and D E09517 .

Keywords

Actinobacillus actinomycetemcomitans
bacterial toxin
immunohistochemical localization
molecular cloning
transport protein
virulence factor

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title = "Structure and function of the B and D genes of the Actinobacillus actinomycetemcomitans leukotoxin complex",

abstract = "The Actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the AaLtC and AaLtA genes reported. The present paper details the sequences of the AaLtB and AaLtD genes which, like AaLtC and AaLtA, are also homologues of genes found in other cytolytic toxin complexes of several other Gram-negative bacterial pathogens. When tested in a recombinant expression system, the AaLtB and/or AaLtD genes are required for the translocation and insertion of the A. actinomycetemcomitans leukotoxin (AaLtA) into the cell membrane of Escherichia coli.",

keywords = "Actinobacillus actinomycetemcomitans, bacterial toxin, immunohistochemical localization, molecular cloning, transport protein, virulence factor",

author = "Lally, {Edward T.} and Golub, {Ellis E.} and Kieba, {Irene R.} and Taichman, {Norton S.} and Sylvia Decker and Peter Berthold and Gibson, {Carolyn W.} and Demuth, {Donald R.} and Joel Rosenbloom",

note = "Funding Information: This work was supported by National Institutes of Health grants DE07118, DE08239 and D E09517 .",

year = "1991",

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doi = "10.1016/0882-4010(91)90004-T",

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AU - Lally, Edward T.

AU - Golub, Ellis E.

AU - Kieba, Irene R.

AU - Taichman, Norton S.

AU - Decker, Sylvia

AU - Berthold, Peter

AU - Gibson, Carolyn W.

AU - Demuth, Donald R.

AU - Rosenbloom, Joel

N1 - Funding Information: This work was supported by National Institutes of Health grants DE07118, DE08239 and D E09517 .

PY - 1991/8

Y1 - 1991/8

N2 - The Actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the AaLtC and AaLtA genes reported. The present paper details the sequences of the AaLtB and AaLtD genes which, like AaLtC and AaLtA, are also homologues of genes found in other cytolytic toxin complexes of several other Gram-negative bacterial pathogens. When tested in a recombinant expression system, the AaLtB and/or AaLtD genes are required for the translocation and insertion of the A. actinomycetemcomitans leukotoxin (AaLtA) into the cell membrane of Escherichia coli.

AB - The Actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the AaLtC and AaLtA genes reported. The present paper details the sequences of the AaLtB and AaLtD genes which, like AaLtC and AaLtA, are also homologues of genes found in other cytolytic toxin complexes of several other Gram-negative bacterial pathogens. When tested in a recombinant expression system, the AaLtB and/or AaLtD genes are required for the translocation and insertion of the A. actinomycetemcomitans leukotoxin (AaLtA) into the cell membrane of Escherichia coli.

KW - Actinobacillus actinomycetemcomitans

KW - bacterial toxin

KW - immunohistochemical localization

KW - molecular cloning

KW - transport protein

KW - virulence factor

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Structure and function of the B and D genes of the Actinobacillus actinomycetemcomitans leukotoxin complex

Abstract

Bibliographical note

Keywords

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