TY - JOUR
T1 - Structure elucidation of DNA-protein crosslinks by using reductive desulfurization and liquid chromatography-tandem mass spectrometry
AU - Wickramaratne, Susith
AU - Tretyakova, Natalia Y.
PY - 2014/2/10
Y1 - 2014/2/10
N2 - Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.
AB - Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.
KW - DNA-protein crosslinks
KW - O-alkylguanine DNA alkyltransferase
KW - desulfurization
KW - labile and nonlabile DNA adducts
KW - liquid chromatography-tandem mass spectrometry
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U2 - 10.1002/cbic.201300757
DO - 10.1002/cbic.201300757
M3 - Article
C2 - 24436288
AN - SCOPUS:84895075082
SN - 1439-4227
VL - 15
SP - 353
EP - 355
JO - ChemBioChem
JF - ChemBioChem
IS - 3
ER -