Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT-DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl-DNA adducts, which can be readily characterized by LC-MSn.
- DNA-protein crosslinks
- O-alkylguanine DNA alkyltransferase
- labile and nonlabile DNA adducts
- liquid chromatography-tandem mass spectrometry