Structure of an RNA internal loop consisting of tandem C-A+ base pairs

Se Bok Jang, Li Wei Hung, Young In Chi, Elizabeth L. Holbrook, Richard J. Carter, Stephen R. Holbrook

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

The crystal structure of the RNA octamer 5'-CGC(CA)GCG-3' has been determined from X-ray diffraction data to 2.3 Å resolution. In the crystal, this oligomer forms a self-complementary double helix in the asymmetric unit. Tandem non-Watson-Crick C-A and A-C base pairs comprise an internal loop in the middle of the duplex, which is incorporated with little distortion of the A-form double helix. From the geometry of the C-A base pairs, it is inferred that the adenosine imino group is protonated and donates a hydrogen bond to the carbonyl group of the cytosine. The wobble geometry of the C-A+ base pairs is very similar to that of the common U-G non-Watson-Crick pair.

Original languageEnglish (US)
Pages (from-to)11726-11731
Number of pages6
JournalBiochemistry
Volume37
Issue number34
DOIs
StatePublished - Aug 25 1998

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