Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

Ping Wang, Ruo Rong Cai, You Min Feng, You Shang Zhang

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalIUBMB Life
Volume49
Issue number4
DOIs
StatePublished - 2000

Keywords

  • Growth-promoting functional region
  • Hybrid molecule
  • IGF-1
  • Insulin

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