Abstract
Neonatal rat pancreatic tissue was frozen to -196 °C using Me2SO as a cryoprotectant and a slow freezing rate to -70 °C followed by immersion in liquid nitrogen. Rapid thawing was used. Viability was demonstrated by successful transplantation to streptozotocin-induced diabetic recipients. Long-term preservation, up to 4 weeks, did not demonstrably affect viability. Cryopreservation techniques may afford a method for providing a diabetic recipient the opportunity to receive a large quantity of pooled islet tissue from well-matched donors.
Original language | English (US) |
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Pages (from-to) | 226-229 |
Number of pages | 4 |
Journal | Cryobiology |
Volume | 20 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1983 |