Incubation of bovine splenic lymphoid cells with pokeweed mitogen (PWM) and bovine viral diarrhea (BVD) virus for 5 days caused a consistent, statistically significant decrease in plasma cell development (cells containing intracytoplasmic immunoglobulin) as well as IgG or IgM synthesis compared to cultures without BVD virus. Suppression of plasma cell development and immunoglobulin synthesis was not due to decreased cell viability in virus-infected cultures even though virus replication was evident in both PWM-stimulated as well as control cultures. Finally, it was shown that heat-inactivated BVD virus was not capable of suppressing either plasma cell development or immunoglobulin synthesis. Thus, these data support the hypothesis that BVD virus can alter B-cell responses, plasma cell development, and subsequent synthesis of IgG and IgM immunoglobulins.
Bibliographical noteFunding Information:
Supported in part by the Minnesota Agricultural Experiment Station and the Cooperative Research Service, U.S. Department of Agriculture. The authors wish to thank Ellen D. Sloane and David R. Senogles for helpful assistance.
Copyright 2014 Elsevier B.V., All rights reserved.