Suppression of inducible nitric oxide synthase by 10-23 DNAzymes in murine macrophage

Indrajit Chaudhury; Sunil K. Raghav; Hemant K. Gautam; Hasi R. Das; Rakha H. Das

doi:10.1016/j.febslet.2006.03.004

Suppression of inducible nitric oxide synthase by 10-23 DNAzymes in murine macrophage

Indrajit Chaudhury, Sunil K. Raghav, Hemant K. Gautam, Hasi R. Das, Rakha H. Das

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Abstract

iNOS mRNA of J774 murine macrophage cells was cleaved by 10-23 DNAzymes. DNAzyme target site I or translation initiation site and site II have computer predicted (MFOLD) secondary structures but site III has no secondary structure. All the three DNAzymes cleaved the short transcripts generated from cloned DNA almost with equal efficiency while cleavage efficiency is higher at site III than the other two sites on isolated iNOS mRNA. Interestingly, at intracellular level, DNAzyme targeted at translation initiation codon (site I) having secondary structure cleaved iNOS mRNA, and suppressed its activity and protein expression more efficiently than that targeted at sites II and III.

Original language	English (US)
Pages (from-to)	2046-2052
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2006.03.004
State	Published - Apr 3 2006
Externally published	Yes

Keywords

DNAzyme
Macrophage
Nitric oxide

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title = "Suppression of inducible nitric oxide synthase by 10-23 DNAzymes in murine macrophage",

abstract = "iNOS mRNA of J774 murine macrophage cells was cleaved by 10-23 DNAzymes. DNAzyme target site I or translation initiation site and site II have computer predicted (MFOLD) secondary structures but site III has no secondary structure. All the three DNAzymes cleaved the short transcripts generated from cloned DNA almost with equal efficiency while cleavage efficiency is higher at site III than the other two sites on isolated iNOS mRNA. Interestingly, at intracellular level, DNAzyme targeted at translation initiation codon (site I) having secondary structure cleaved iNOS mRNA, and suppressed its activity and protein expression more efficiently than that targeted at sites II and III.",

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AU - Chaudhury, Indrajit

AU - Raghav, Sunil K.

AU - Gautam, Hemant K.

AU - Das, Hasi R.

AU - Das, Rakha H.

PY - 2006/4/3

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AB - iNOS mRNA of J774 murine macrophage cells was cleaved by 10-23 DNAzymes. DNAzyme target site I or translation initiation site and site II have computer predicted (MFOLD) secondary structures but site III has no secondary structure. All the three DNAzymes cleaved the short transcripts generated from cloned DNA almost with equal efficiency while cleavage efficiency is higher at site III than the other two sites on isolated iNOS mRNA. Interestingly, at intracellular level, DNAzyme targeted at translation initiation codon (site I) having secondary structure cleaved iNOS mRNA, and suppressed its activity and protein expression more efficiently than that targeted at sites II and III.

KW - DNAzyme

KW - Macrophage

KW - Nitric oxide

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Suppression of inducible nitric oxide synthase by 10-23 DNAzymes in murine macrophage

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