Surface modification and bioconjugation of FeCo magnetic nanoparticles with proteins

Wei Wang, Ying Jing, Shihai He, Jian Ping Wang, Jian Ping Zhai

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Magnetic Fe70Co30 nanoparticles with a cubic shape and a mean size of 15±1.5nm were fabricated using a magnetron-sputtering-based gas phase condensation deposition method. The particles had a high saturation magnetization of 220emu/g, which is much higher than that of commercially available iron oxide nanoparticles. The FeCo nanoparticles were modified by 3-aminopropyltriethoxy silane and subsequently activated by glutaraldehyde, leading to successful attachment of aldehyde groups onto nanoparticle surfaces. Three proteins, namely streptavidin, PAPP-A antibody and Nectin-4 antibody, were immobilized on glutaraldehyde activated FeCo nanoparticles, and their loading levels were quantitatively evaluated. Our results show that loading capabilities are 95μg of streptavidin, 128μg of PAPP-A, and 125μg of Nectin-4 antibody per milligram of FeCo nanoparticles, and that the three immobilized proteins retain their binding bioactivity. The protein-FeCo conjugates may find valuable applications involving magnetic separation and purification of proteins and cells, and the magnetic detection of biomolecules.

Original languageEnglish (US)
Pages (from-to)449-456
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume117
DOIs
StatePublished - May 1 2014

Keywords

  • FeCo magnetic nanoparticles
  • Immobilization
  • Protein
  • Surface modification

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