Surface Plasmon Resonance Study of the Binding of PEO-PPO-PEO Triblock Copolymer and PEO Homopolymer to Supported Lipid Bilayers

Mihee Kim, Milan Vala, Christopher T. Ertsgaard, Sang Hyun Oh, Timothy P. Lodge, Frank S. Bates, Benjamin J. Hackel

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Poloxamer 188 (P188), a poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) triblock copolymer, protects cell membranes against various external stresses, whereas poly(ethylene oxide) (PEO; 8600 g/mol) homopolymer lacks protection efficacy. As part of a comprehensive effort to elucidate the protection mechanism, we used surface plasmon resonance (SPR) to obtain direct evidence of binding of the polymers onto supported lipid bilayers. Binding kinetics and coverage of P188 and PEO were examined and compared. Most notably, PEO exhibited membrane association comparable to that of P188, evidenced by comparable association rate constants and coverage. This result highlights the need for additional mechanistic understanding beyond simple membrane association to explain the differential efficacy of P188 in therapeutic applications.

Original languageEnglish (US)
Pages (from-to)6703-6712
Number of pages10
JournalLangmuir
Volume34
Issue number23
DOIs
StatePublished - Jun 12 2018

Bibliographical note

Funding Information:
This work was supported by a grant from the National Institutes of Health (R01 HL122323) to F.S.B. M.V., C.T.E., and S.-H.O. acknowledge support from the Minnesota Partnership for Biotechnology and Medical Genomics. SPR chips were fabricated in the Minnesota Nano Center (MNC) cleanroom, which receives partial support from the National Science Foundation (NSF) through the National Nanotechnology Coordinated Infrastructure (NNCI).

Publisher Copyright:
© 2018 American Chemical Society.

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