TY - JOUR
T1 - Symbiont evolution during the free-living phase can improve host colonization
AU - Soto, William
AU - Travisano, Michael
AU - Tolleson, Alexandra Rose
AU - Nishiguchi, Michele Kiyoko
N1 - Publisher Copyright:
© 2019, Microbiology Society. All rights reserved.
PY - 2019/2
Y1 - 2019/2
N2 - For micro-organisms cycling between free-living and host-associated stages, where reproduction occurs in both of these lifestyles, an interesting inquiry is whether evolution during the free-living stage can be positively pleiotropic to microbial fitness in a host environment. To address this topic, the squid host Euprymna tasmanica and the marine bioluminescent bacterium Vibrio fischeri were utilized. Microbial ecological diversification in static liquid microcosms was used to simulate symbiont evolution during the free-living stage. Thirteen genetically distinct V. fischeri strains from a broad diversity of ecological sources (e.g. squid light organs, fish light organs and seawater) were examined to see if the results were reproducible in many different genetic settings. Genetic backgrounds that are closely related can be predisposed to considerable differences in how they respond to similar selection pressures. For all strains examined, new mutations with striking and facilitating effects on host colonization arose quickly during microbial evolution in the free-living stage, regardless of the ecological context under consideration for a strain’s genetic background. Microbial evolution outside a host environment promoted host range expansion, improved host colonization for a micro-organism, and diminished the negative correlation between biofilm formation and motility.
AB - For micro-organisms cycling between free-living and host-associated stages, where reproduction occurs in both of these lifestyles, an interesting inquiry is whether evolution during the free-living stage can be positively pleiotropic to microbial fitness in a host environment. To address this topic, the squid host Euprymna tasmanica and the marine bioluminescent bacterium Vibrio fischeri were utilized. Microbial ecological diversification in static liquid microcosms was used to simulate symbiont evolution during the free-living stage. Thirteen genetically distinct V. fischeri strains from a broad diversity of ecological sources (e.g. squid light organs, fish light organs and seawater) were examined to see if the results were reproducible in many different genetic settings. Genetic backgrounds that are closely related can be predisposed to considerable differences in how they respond to similar selection pressures. For all strains examined, new mutations with striking and facilitating effects on host colonization arose quickly during microbial evolution in the free-living stage, regardless of the ecological context under consideration for a strain’s genetic background. Microbial evolution outside a host environment promoted host range expansion, improved host colonization for a micro-organism, and diminished the negative correlation between biofilm formation and motility.
KW - Bioluminescence
KW - Ecological diversification
KW - Host-microbe interactions
KW - Symbiosis
UR - http://www.scopus.com/inward/record.url?scp=85061150776&partnerID=8YFLogxK
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U2 - 10.1099/mic.0.000756
DO - 10.1099/mic.0.000756
M3 - Article
C2 - 30648935
AN - SCOPUS:85061150776
SN - 1350-0872
VL - 165
SP - 174
EP - 187
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
IS - 2
M1 - 000756
ER -