Synergistic activation of transcription by bacteriophage λ cl protein and E. coli cAMP receptor protein

J. Keith Joung, Deanna M. Koepp, Ann Hochschild

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Two heterologous prokaryotic activators, the bacteriophage λ cl protein (λcl) and the Escherichia coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding sites for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. These results imply that both proteins interact directly with RNA polymerase and thus suggest a precise mechanism for transcriptional synergy: the interaction of two activators with two distinct surfaces of RNA polymerase.

Original languageEnglish (US)
Pages (from-to)1863-1866
Number of pages4
JournalScience
Volume265
Issue number5180
DOIs
StatePublished - 1994

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