Abstract
Cells are highly sensitive to topographic and mechanical features of the surrounding extracellular matrix (ECM) environment. Indeed, cells respond to these physical cues to coordinate adhesion-mediated signaling and the dynamic cytoskeletal remodeling underlying locomotion. Inspired by this biophysical relationship between cells and the ECM, particularly within the tumor microenvironment, we engineered an innovative biomimetic platform using substrate nanotopography and surface modification techniques in order to guide and promote the migration of oncogenic PIK3CA knockin mutants compared to their wild type non-transformed counterpart, MCF-10A human breast epithelial cells. Results demonstrate that human mammary epithelial cells collectively interact with the engineered microstructural environment, which regulates cell migration with increased velocity and persistence time.
| Original language | English (US) |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 221-223 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: Oct 25 2015 → Oct 29 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 10/25/15 → 10/29/15 |
Bibliographical note
Funding Information:This work was supported by the new faculty startup fund of the department of bioengineering at the university of Washington, and the FHCRC/UW Cancer Consortium Cancer Center Support Grant of the National Institutes of Health under Award Number P30 CA015704. It was also partly supported by funds from the College of Science and Engineering and the Masonic Cancer Center of the University of Minnesota, grants from the UMN Institute for Engineering in Medicine, and UMN/UAB Pancreatic Cancer SPORE, and Institute for Basic Science (IBS) and the Pioneer Research Center Program (NRF-2012-0009555), and the Korea Basic Science Institute (KBSI) grant (D35500).
Publisher Copyright:
© 15CBMS-0001.
Keywords
- Capillary force lithography
- Cell patterning
- Contact-guided cell migration
- MCF-10A
- Nanotopography
- Oncogenic PIK3CA knockin mutations
- Plasma lithography
