Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Hao Luo; Lianjia Ma; Yu Zhang; Peter W. Carr

doi:10.1016/j.chroma.2007.11.104

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Hao Luo, Lianjia Ma, Yu Zhang, Peter W. Carr

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A novel type of silica-based sulfonate-modified reversed phase (^-SO₃-HC-C₈) has been synthesized; it is based on a newly developed acid stable "hyper-crosslinked" C₈ derivatized reversed phase, denoted HC-C₈. The ^-SO₃-HC-C₈ phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C₈ phase at different temperatures. The ^-SO₃-HC-C₈ phases are only slightly less hydrophobic than the parent HC-C₈ phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown through Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the very high values of Snyder's column comparison function F_s. In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity of these ^-SO₃-HC-C₈ phases is greatly enhanced by its high hydrophobicity through a "hydrophobically assisted" ion-exchange retention process.

Original language	English (US)
Pages (from-to)	41-55
Number of pages	15
Journal	Journal of Chromatography A
Volume	1182
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2007.11.104
State	Published - Feb 22 2008

Bibliographical note

Funding Information:
We thank the National Institutes of Health for the financial support. We also thank Agilent Technologies Inc. (Wilmington, DE, USA) for the donation of Zorbax silica and SB C 18 phases.

Keywords

Basic analytes
Cation exchange
HPLC
Reversed phase
Selectivity
Stationary phase

Access

10.1016/j.chroma.2007.11.104

OpenUrl availability

Full text

Cite this

@article{e3885544606c4ff788fcf417bc4426a6,

title = "Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases",

abstract = "A novel type of silica-based sulfonate-modified reversed phase (-SO3-HC-C8) has been synthesized; it is based on a newly developed acid stable {"}hyper-crosslinked{"} C8 derivatized reversed phase, denoted HC-C8. The -SO3-HC-C8 phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C8 phase at different temperatures. The -SO3-HC-C8 phases are only slightly less hydrophobic than the parent HC-C8 phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown through Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the very high values of Snyder's column comparison function Fs. In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity of these -SO3-HC-C8 phases is greatly enhanced by its high hydrophobicity through a {"}hydrophobically assisted{"} ion-exchange retention process.",

keywords = "Basic analytes, Cation exchange, HPLC, Reversed phase, Selectivity, Stationary phase",

author = "Hao Luo and Lianjia Ma and Yu Zhang and Carr, {Peter W.}",

note = "Funding Information: We thank the National Institutes of Health for the financial support. We also thank Agilent Technologies Inc. (Wilmington, DE, USA) for the donation of Zorbax silica and SB C 18 phases.",

year = "2008",

month = feb,

day = "22",

doi = "10.1016/j.chroma.2007.11.104",

language = "English (US)",

volume = "1182",

pages = "41--55",

journal = "Journal of Chromatography A",

issn = "0021-9673",

number = "1",

}

TY - JOUR

T1 - Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

AU - Luo, Hao

AU - Ma, Lianjia

AU - Zhang, Yu

AU - Carr, Peter W.

N1 - Funding Information: We thank the National Institutes of Health for the financial support. We also thank Agilent Technologies Inc. (Wilmington, DE, USA) for the donation of Zorbax silica and SB C 18 phases.

PY - 2008/2/22

Y1 - 2008/2/22

N2 - A novel type of silica-based sulfonate-modified reversed phase (-SO3-HC-C8) has been synthesized; it is based on a newly developed acid stable "hyper-crosslinked" C8 derivatized reversed phase, denoted HC-C8. The -SO3-HC-C8 phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C8 phase at different temperatures. The -SO3-HC-C8 phases are only slightly less hydrophobic than the parent HC-C8 phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown through Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the very high values of Snyder's column comparison function Fs. In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity of these -SO3-HC-C8 phases is greatly enhanced by its high hydrophobicity through a "hydrophobically assisted" ion-exchange retention process.

AB - A novel type of silica-based sulfonate-modified reversed phase (-SO3-HC-C8) has been synthesized; it is based on a newly developed acid stable "hyper-crosslinked" C8 derivatized reversed phase, denoted HC-C8. The -SO3-HC-C8 phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C8 phase at different temperatures. The -SO3-HC-C8 phases are only slightly less hydrophobic than the parent HC-C8 phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown through Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the very high values of Snyder's column comparison function Fs. In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity of these -SO3-HC-C8 phases is greatly enhanced by its high hydrophobicity through a "hydrophobically assisted" ion-exchange retention process.

KW - Basic analytes

KW - Cation exchange

KW - HPLC

KW - Reversed phase

KW - Selectivity

KW - Stationary phase

UR - http://www.scopus.com/inward/record.url?scp=38549120300&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38549120300&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2007.11.104

DO - 10.1016/j.chroma.2007.11.104

M3 - Article

C2 - 18207150

AN - SCOPUS:38549120300

SN - 0021-9673

VL - 1182

SP - 41

EP - 55

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1

ER -

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this