The development of faster and less expensive methods to discover bioactive small molecules remains a high priority in chemical biology. This article discusses one alternative to traditional high-throughput screening: the synthesis and screening of one bead one compound (OBOC) libraries. Protocols are provided to create and screen libraries of peptoid displayed on TentaGel beads, which is a cheap and relatively straightforward process for the identification of selective protein ligands. However, peptoids bind to proteins with modest affinity in most cases. Therefore, we also describe protocols to create libraries of stiffer oligomers called PICCOs (peptoid-inspired, conformationally constrained oligomers) that have proven to be a superior source of high affinity ligands.
|Original language||English (US)|
|Title of host publication||Methods in Enzymology|
|Editors||Arun K. Shukla|
|Publisher||Academic Press Inc.|
|Number of pages||37|
|State||Published - 2019|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
The National Institutes of Health (AG 054892) supported some of the work described here.
© 2019 Elsevier Inc.
- High-throughput screening
- One bead one compound libraries
- Solid-phase synthesis