TY - JOUR
T1 - Synthesis and sequence preference of acetaldehyde-derived oligonucleotide interstrand cross-link
AU - Lao, Yanbin
AU - Moriya, Masaaki
AU - Hecht, Stephen S.
PY - 2004
Y1 - 2004
N2 - Acetaldehyde (AA) is mutagenic and carcinogenic. Several previously unknown AA-DNA adducts have been identified, including an interstrand cross-link, 3-(2-deoxyribos-1-yl)-5, 6,7,8-tetrahydro-8-(N2-deoxyguanosyl)-6-methylpyrimido [1, 2-a]purine-10(3H)one. This cross-link was synthesized in a double-stranded oligonucleotide appropriate for studies of mutagenicity and repair in human cells. The cross-linked oligonucleotide was characterized by MS and enzymatic hydrolysis experiments. Another oligonucleotide containing the irreversible analog of the cross-link, 1,3-bis(2x-deoxyguanos-N2-yl) butane, was also synthesized and characterized. It is being utilized as a control in the mutagenicity studies. The sequence preference for AA-derived cross-link formation was also determined. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).
AB - Acetaldehyde (AA) is mutagenic and carcinogenic. Several previously unknown AA-DNA adducts have been identified, including an interstrand cross-link, 3-(2-deoxyribos-1-yl)-5, 6,7,8-tetrahydro-8-(N2-deoxyguanosyl)-6-methylpyrimido [1, 2-a]purine-10(3H)one. This cross-link was synthesized in a double-stranded oligonucleotide appropriate for studies of mutagenicity and repair in human cells. The cross-linked oligonucleotide was characterized by MS and enzymatic hydrolysis experiments. Another oligonucleotide containing the irreversible analog of the cross-link, 1,3-bis(2x-deoxyguanos-N2-yl) butane, was also synthesized and characterized. It is being utilized as a control in the mutagenicity studies. The sequence preference for AA-derived cross-link formation was also determined. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).
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M3 - Conference article
AN - SCOPUS:5044244673
SN - 0065-7727
VL - 228
SP - TOXI-18
JO - ACS National Meeting Book of Abstracts
JF - ACS National Meeting Book of Abstracts
IS - 1
T2 - Abstracts of Papers - 228th ACS National Meeting
Y2 - 22 August 2004 through 26 August 2004
ER -