Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins; Kevin L. Peterson; Thomas G. Beito; Karen S. Flatten; Scott H. Kaufmann; Daniel A. Harki

doi:10.1039/c5ob02049b

Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Angela L. Perkins, Kevin L. Peterson, Thomas G. Beito, Karen S. Flatten, Scott H. Kaufmann, Daniel A. Harki

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Original language	English (US)
Pages (from-to)	4103-4109
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Volume	14
Issue number	17
DOIs	https://doi.org/10.1039/c5ob02049b
State	Published - 2016

Bibliographical note

Funding Information:
This work was supported by a grant from the Minnesota Partnership for Biotechnology and Medical Genomics (#10.03 to DAH & SHK). Mass spectrometry was performed at the Analytical Biochemistry Core Facility of the University of Minnesota Masonic Cancer Center, which is supported by the NIH (P30-CA77598).

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1039/c5ob02049b

http://europepmc.org/articles/pmc4922793

OpenUrl availability

Full text

Cite this

@article{4e627df347444c92a47748a92b49a77e,

title = "Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes",

abstract = "The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.",

author = "Perkins, {Angela L.} and Peterson, {Kevin L.} and Beito, {Thomas G.} and Flatten, {Karen S.} and Kaufmann, {Scott H.} and Harki, {Daniel A.}",

note = "Funding Information: This work was supported by a grant from the Minnesota Partnership for Biotechnology and Medical Genomics (#10.03 to DAH & SHK). Mass spectrometry was performed at the Analytical Biochemistry Core Facility of the University of Minnesota Masonic Cancer Center, which is supported by the NIH (P30-CA77598). Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry.",

year = "2016",

doi = "10.1039/c5ob02049b",

language = "English (US)",

volume = "14",

pages = "4103--4109",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

publisher = "Royal Society of Chemistry",

number = "17",

}

TY - JOUR

T1 - Synthesis of a peptide-universal nucleotide antigen

T2 - Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

AU - Perkins, Angela L.

AU - Peterson, Kevin L.

AU - Beito, Thomas G.

AU - Flatten, Karen S.

AU - Kaufmann, Scott H.

AU - Harki, Daniel A.

N1 - Funding Information: This work was supported by a grant from the Minnesota Partnership for Biotechnology and Medical Genomics (#10.03 to DAH & SHK). Mass spectrometry was performed at the Analytical Biochemistry Core Facility of the University of Minnesota Masonic Cancer Center, which is supported by the NIH (P30-CA77598). Publisher Copyright: © 2016 The Royal Society of Chemistry.

PY - 2016

Y1 - 2016

N2 - The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

AB - The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

UR - http://www.scopus.com/inward/record.url?scp=84973322839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973322839&partnerID=8YFLogxK

U2 - 10.1039/c5ob02049b

DO - 10.1039/c5ob02049b

M3 - Article

C2 - 27113574

AN - SCOPUS:84973322839

SN - 1477-0520

VL - 14

SP - 4103

EP - 4109

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 17

ER -

Synthesis of a peptide-universal nucleotide antigen: Towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Abstract

Bibliographical note

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this